Measured Retention Times for Preloaded Data Sets
There are a total of 10 sets of experimental retention times preloaded into the software. They were acquired from 5 different
experimental conditions run on two different HPLC instruments.
The experimental retention times of the gradient calibration solutes can be automatically entered by clicking
on the "Use Preloaded Values" button and selecting a dataset. The experimental retention times of the other
database solutes are shown below. You can use these retention times to check the accuracy of retention times predicted from back-calculated gradient and flow rate profiles.
aN.D. means not determined.
Primary Instrument: Thermo Scientific Inc. Accela UHPLC Pump
Condition 1: 10% B to 100% B in 5 min at 200 μL/min

Condition 2: 10% B to 100% B in 20 min at 100 μL/min

Condition 3: 10% B to 100% B in 20 min at 200 μL/min

Condition 4: 10% B to 100% B in 20 min at 400 μL/min

Condition 5: 10% B to 100% B in 80 min at 200 μL/min

Secondary Instrument: Waters Corporation Acquity UPLC Pump
Condition 1: 10% B to 100% B in 5 min at 200 μL/min

Condition 2: 10% B to 100% B in 20 min at 100 μL/min

Condition 3: 10% B to 100% B in 20 min at 200 μL/min

Condition 4: 10% B to 100% B in 20 min at 400 μL/min

Condition 5: 10% B to 100% B in 80 min at 200 μL/min
